AB0020 CYTOCHEMICAL ASSESSMENT IN TREG CELLS OF PSORIASIC ARTHROPATHY BY INHIBITION OF IL23 (IN REAL LIFE) AND ITS CORRELATION WITH CLINICAL REMISSION

Background Psoriatic arthropathy (PsA) is a heterogeneous chronic entity with multiple clinical domains that evolves to irreversible damage both organic and psychological. Therapeutic recommendation guidelines advise differentiated therapies according the dominant domain (e.g., peripheral arthritis, dactylitis, enthesitis, spondylitis, cutaneous or nail psoriasis). Moderate-severe involvement requires use of targeted biological synthetic medication. Primary secondary failures intolerances often force change therapeutic target in order achieve greater efficiency. The interrelation cytokines (mainly lL23, IL17, IL10) their pathogenesis at “in vitro” level seem proven, but modification vivo” correlation clinic are little studied. Objectives To identify cytokine profile IL -10 -17 CD4+FoxP3+ cells patients PsA, (ii) degree expression CD152 PsA (iii) correlate these molecules state Minimal Activity disease (MDA). Methods present study involved 9 healthy volunteers 10 Arthritis (CASPAR criteria) treatment for least 6 months anti-IL23 remission analyze production IL-17A IL-10 by regulatory T under basal culture stimulated conditions (1 μg/ml Ionomycin (Fisher Scientific, EU) 25 ng/ml forbol-12-myristate-13-acetate (PMA) (MP Biomedicals, EU)., monoclonal antibodies were used: anti-CD4-FITC, anti-CD25-PECy5, anti-FoxP3-PE, anti-IL-10-PECy7 anti-IL-17A-APC (eBioscience, EU), processed flow cytometry (FACS Canto, BD Bioscience, EU). Results Percentage Tregs expressing control condition Healthy: 1.787% BP: 1.418% P value = 0.7354. Healthy condition: 3.178% PA: 2.256% 0.0108. 1.617% AP: 1.665% 0.8923. 6.298% 3.742% 0.0350 Conclusion Although controls expressed similar percentage IL10 IL17 baseline, same does not occur after stimulation culture, showing an increase populations (IL10-IL17) did ArP remission. This differs from RA which increased, as different immunological mechanisms pathologies. Likewise, Treg phenotype has been described double marker RORγt/ FOXP3 production. plasticity T-cell compartment allows immune response adapt local environment. further emphasized recent recognition themselves can differentiate into IL-17-producing cells, particularly when exogenous IL-1β, IL-23, IL-21. In psoriatic levels increased. inhibition IL23 would be suppressing this hence lack (Il10-IL17) References [1]Koenen HJ, Smeets RL, Vink PM, Rijsses Evan, Boots AM, Joosten I. Human CD25highFoxp3pos cells. Sangre. 2008; 112:2340–2352 [2]Microbes Infect. 2009 Apr; 11(5): 594–598. Regulation Treg/Th17 Differentiation Steven F. Ziegler 1 Jane H. Buckner 2 [3]Szodoray P, Alex Chappell-Woodward CM, et al. Circulating Norwegian arthritis determined multiplex array system. Rheumatology (Oxford ) 2007;46:417–25. [4]Davide SimoneSingle cell analysis spondyloarthritis identifies distinct synovial gene patterns clonal fates Commun Biol. 2021; 4: 1395 Acknowledgements: NIL. Disclosure Interests None Declared.